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DNA degradation of Anopheles darlingi collected at high relative humidity and preserved in isopropanol
Rivero, José; Zoghbi, Normig; Rubio-Palis, Yasmin; Urdaneta, Ludmel; Herrera, Flor.
Affiliation
  • Rivero, José; Universidad de Carabobo Núcleo Aragua. Facultad de Ciencias de la Salud. Instituto de Investigaciones Biomédicas. Maracay. VE
  • Zoghbi, Normig; Universidad de Carabobo Núcleo Aragua. Facultad de Ciencias de la Salud. Insituto de Investigaciones Biomédicas. Maracay. VE
  • Rubio-Palis, Yasmin; Ministerio de Salud y Desarrollo Social. Dirección de Salud Ambiental y Contraloría Sanitaria. Maracay. VE
  • Urdaneta, Ludmel; Colorado State University. Department of Microbiology. Colorado. US
  • Herrera, Flor; Universidad de Carabobo Núcleo Aragua. Facultad de Ciencias de la Salud. Instituto de Investigaciones Biomédicas. Maracay. VE
Bol. malariol. salud ambient ; 47(1): 149-151, 2007. ilus
Article in En | LILACS | ID: lil-503713
Responsible library: VE1.1
RESUMEN
Anopheles darlingi mosquitoes, exposed to variable relative humidity (RH) in the field, were preserved during transportation to the laboratory using two different

methods:

100% isopropanol at ambient temperature or frozen in liquid nitrogen or dry ice. The DNA isolated from samples collected at RH greater than 91% and preserved in isopropanol was degraded, while DNA isolated from insects kept in liquid nitrogen or dry ice maintained its integrity when collected under conditions of up to 95% RH.
Subject(s)
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Collection: 01-internacional Database: LILACS Main subject: DNA / Anopheles / Culicidae Country/Region as subject: America do sul / Venezuela Language: En Journal: Bol. malariol. salud ambient Journal subject: CIENCIAS SOCIAIS / MEDICINA TROPICAL Year: 2007 Document type: Article Affiliation country: United States / Venezuela Country of publication: Venezuela
Search on Google
Add to My VHL
Print
XML
Collection: 01-internacional Database: LILACS Main subject: DNA / Anopheles / Culicidae Country/Region as subject: America do sul / Venezuela Language: En Journal: Bol. malariol. salud ambient Journal subject: CIENCIAS SOCIAIS / MEDICINA TROPICAL Year: 2007 Document type: Article Affiliation country: United States / Venezuela Country of publication: Venezuela