Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
Electron. j. biotechnol
; 10(4): 627-632, oct. 2007. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-504113
Responsible library:
CL1.1
ABSTRACT
Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica serovar Enteritidis (hereafter S. Enteritidis) were avirulent in the Caenorhabditis elegans animal model. We isolated phage-resistant strains of S. Enteritidis ATCC 13076 by using three lytic phages (f2aSE, f3aSE and f18aSE). In these mutants, we explored different virulence factors like lipopolysaccharide (LPS), virulence plasmid (Pla), motility and type I fimbriae, all of which may have effects on virulence and could furthermore be related to phage resistance. The phage-resistant strains of S. Enteritidis showed loss of O-Polysaccharide (O-PS) and auto-agglutination, present a rough phenotype and consequently they are avirulent in the C. elegans animal model. We speculate that the O-PS is necessary for phage attachment to the S. Enteritidis cell surface.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
LILACS
Main subject:
Salmonella enteritidis
/
Bacteriophages
/
Lipopolysaccharides
Type of study:
Etiology study
/
Prognostic study
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2007
Document type:
Article
Affiliation country:
Chile
/
United States
Institution/Affiliation country:
Arizona State University/US
/
Pontificia Universidad Católica de Valparaíso/CL