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Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
Azadi, Pejman; Khosh-Khui, Morteza.
Affiliation
  • Azadi, Pejman; National Research Center of Ornamental Plants. Tissue Culture Department. Mahallat. IR
  • Khosh-Khui, Morteza; Shiraz University. College of Agriculture. Department of Horticultural Science. Shiraz. IR
Electron. j. biotechnol ; Electron. j. biotechnol;10(4): 582-591, oct. 2007. ilus, graf, tab
Article in En | LILACS | ID: lil-504117
Responsible library: CL1.1
ABSTRACT
A protocol for the micropropagation in different harvesting time of Lilium ledebourii (Baker) Boiss, an endangered rare species endemic to Iran has been developed. In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. Among the various treatments tested, the Murashige and Skoog (MS) medium supplemented with 0.1 mg l-1 naphthaleneacetic acid (NAA) + 0.1 mg l-1 benzyladenin (BA) and 6% sucrose in all harvesting seasons proved to be superior to others. The best results for fresh weight of bulblets, rooting parameters and the survival rate after transplantation to greenhouse were obtained from early winter-harvested bulbs. Summer-harvested bulbs had the highest number of bulblets per explant. The bulblets at the end of the culture period were given cold treatment at 4ºC for 2-8 weeks at a 2-weeks interval and then transplanted to a potting mixture of sand, leaf mold and peat moss (111 v/v). The best emergence rate (90%) was achieved at 8 weeks cold treatment for winter harvested bulbs.
Subject(s)
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Cryopreservation / Lilium Type of study: Guideline Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2007 Document type: Article Affiliation country: Iran Country of publication: Chile
Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Cryopreservation / Lilium Type of study: Guideline Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2007 Document type: Article Affiliation country: Iran Country of publication: Chile