Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil
Braz. j. infect. dis
; 13(1): 47-52, Feb. 2009. graf, tab
Article
in English
| LILACS
| ID: lil-517814
Responsible library:
BR1.1
ABSTRACT
We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3 percent of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95 percent sensitivity for homologous antigens. Immunoblotting revealed specific proteins for diagnosis of CL and detected 96.6 percent of the patients when L. (V.) braziliensis was used as an antigen, and 83.3 percent with L. (L.) amazonensis. This study demonstrated the importance of differential diagnosis for leishmaniasis; the association of two or more indirect methods increased diagnosis sensitivity.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Leishmania braziliensis
/
Leishmania mexicana
/
Leishmaniasis, Cutaneous
Type of study:
Diagnostic study
/
Evaluation study
/
Prognostic study
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2009
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Prefeitura Municipal de Prudentópolis/BR
/
SESA/BR
/
Universidade Federal do Paraná/BR