Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil
Braz. j. med. biol. res
; 42(10): 877-881, Oct. 2009. ilus
Article
in English
| LILACS
| ID: lil-526184
Responsible library:
BR1.1
ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a total of 8 different pulsed-field gel electrophoresis patterns (types A to H) with predominance (73 percent) of pattern A (BEC-related). However, MRSA harboring SCCmec type IV were also identified, 3 (7 percent) of which were genetically related to the pediatric clone - USA800 (ST5-SCCmecIV). In addition, we found a considerable genetic diversity within BEC isolates. MRSA displaying SCCmecIV are frequently susceptible to the majority of non-β-lactam antibiotics. However, emergence of multiresistant variants of USA800 was detected.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Genetic Variation
/
Methicillin-Resistant Staphylococcus aureus
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2009
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Norte/BR
/
Universidade Federal do Rio de Janeiro/BR