Your browser doesn't support javascript.
loading
Effects of L1-ORF2 fragments on green fluorescent protein gene expression
Wang, Xiu-Fang; Jin, Xia; Wang, Xiaoyan; Liu, Jing; Feng, Jingjing; Yang, QinQing; Mu, Wenli; Shi, Xiaojuan; Lu, Zhanjun.
Affiliation
  • Wang, Xiu-Fang; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Jin, Xia; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Wang, Xiaoyan; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Liu, Jing; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Feng, Jingjing; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Yang, QinQing; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Mu, Wenli; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Shi, Xiaojuan; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
  • Lu, Zhanjun; Hebei Medical University. Department of Genetics. Hebei Key Lab of Laboratory Animal. Shijiazhuang. CN
Genet. mol. biol ; Genet. mol. biol;32(4): 688-696, 2009. ilus, tab
Article in En | LILACS | ID: lil-531810
Responsible library: BR1.1
ABSTRACT
The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 and ORF2, that code for an RNA-binding protein and a protein with endonuclease and reverse transcriptase activities, respectively. In this work, we examined the effects of full length L1-ORF2 and ORF2 fragments on green fluorescent protein gene (GFP) expression when inserted into the pEGFP-C1 vector downstream of GFP. All of the ORF2 fragments in sense orientation inhibited GFP expression more than when in antisense orientation, which suggests that small ORF2 fragments contribute to the distinct inhibitory effects of this ORF on gene expression. These results provide the first evidence that different 280-bp fragments have distinct effects on the termination of gene transcription, and that when inserted in the antisense direction, fragment 280-9 (the 3' end fragment of ORF2) induces premature termination of transcription that is consistent with the effect of ORF2.
Key words
Full text: 1 Collection: 01-internacional Database: LILACS Language: En Journal: Genet. mol. biol Journal subject: GENETICA Year: 2009 Document type: Article Affiliation country: China Country of publication: Brazil
Full text: 1 Collection: 01-internacional Database: LILACS Language: En Journal: Genet. mol. biol Journal subject: GENETICA Year: 2009 Document type: Article Affiliation country: China Country of publication: Brazil