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Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
Ghorbanpur, M; Mirakabadi, Abbas Zare; Zokaee, F; Zolfagarrian, H.
Affiliation
  • Ghorbanpur, M; Amirkabir University. Chemical Engineering Department. Tehran. IR
  • Mirakabadi, Abbas Zare; Razi Vaccine and Serum Research Institute. Department of Venomous Animals and Antivenom Production. Karaj. IR
  • Zokaee, F; Amirkabir University. Chemical Engineering Department. Tehran. IR
  • Zolfagarrian, H; Razi Vaccine and Serum Research Institute. Department of Venomous Animals and Antivenom Production. Karaj. IR
J. venom. anim. toxins incl. trop. dis ; 16(1): 96-106, 2010. graf, tab, ilus
Article in English | LILACS, VETINDEX | ID: lil-542432
Responsible library: BR68.1
ABSTRACT
An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83 percent. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.(AU)
Subject(s)

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Full text: Available Database: LILACS / VETINDEX Main subject: Chromatography, Ion Exchange / Agkistrodon / Crotalid Venoms / Anticoagulants Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Year: 2010 Document type: Article Institution/Affiliation country: Amirkabir University/IR / Razi Vaccine and Serum Research Institute/IR
Fulltext
Add to My VHL
Print
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Full text: Available Database: LILACS / VETINDEX Main subject: Chromatography, Ion Exchange / Agkistrodon / Crotalid Venoms / Anticoagulants Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Year: 2010 Document type: Article Institution/Affiliation country: Amirkabir University/IR / Razi Vaccine and Serum Research Institute/IR