Mycobacterium leprae downregulates the expression of PHEX in Schwann cells and osteoblasts
Mem. Inst. Oswaldo Cruz
; 105(5): 627-632, Aug. 2010. ilus, graf
Article
in English
| LILACS, Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: lil-557221
Responsible library:
BR1.1
Localization: BR191.1; 9613/S
ABSTRACT
Neuropathy and bone deformities, lifelong sequelae of leprosy that persist after treatment, result in significant impairment to patients and compromise their social rehabilitation. Phosphate-regulating gene with homologies to endopeptidase on the X chromosome (PHEX) is a Zn-metalloendopeptidase, which is abundantly expressed in osteoblasts and many other cell types, such as Schwann cells, and has been implicated in phosphate metabolism and X-linked rickets. Here, we demonstrate that Mycobacterium leprae stimulation downregulates PHEX transcription and protein expression in a human schwannoma cell line (ST88-14) and human osteoblast lineage. Modulation of PHEX expression was observed to a lesser extent in cells stimulated with other species of mycobacteria, but was not observed in cultures treated with latex beads or with the facultative intracellular bacterium Salmonella typhimurium. Direct downregulation of PHEX by M. leprae could be involved in the bone resorption observed in leprosy patients. This is the first report to describe PHEX modulation by an infectious agent.
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Leprosy
/
Neglected Diseases
Database:
HANSEN
/
Hanseníase Leprosy
/
LILACS
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
/
SESSP-ILSLPROD
Main subject:
Osteoblasts
/
Schwann Cells
/
Leprosy
/
Mycobacterium leprae
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Year:
2010
Document type:
Article
Institution/Affiliation country:
Fiocruz/BR
/
Secretaria de Estado da Saúde/BR
/
Universidade do Estado do Rio de Janeiro/BR