Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes
Mem. Inst. Oswaldo Cruz
; 106(2): 190-193, Mar. 2011. graf, tab
Article
in English
| LILACS
| ID: lil-583944
Responsible library:
BR1.1
ABSTRACT
The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Leishmania mexicana
/
RNA, Messenger
/
RNA, Protozoan
/
Genes, Protozoan
/
Gene Expression Profiling
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Butantan/BR
/
Universidade de São Paulo/BR