In vitro activity of amphotericin B cochleates against Leishmania chagasi
Mem. Inst. Oswaldo Cruz
; 106(2): 251-253, Mar. 2011. graf, tab
Article
in English
| LILACS
| ID: lil-583954
Responsible library:
BR1.1
ABSTRACT
Cochleate delivery vehicles are a novel lipid-based system with potential for delivery of amphotericin B (AmB). In this study, the efficacy of cochleates was evaluated by examining the in vitro activity of AmB cochleates (CAMB) against Leishmania chagasi in a macrophage model of infection. We demonstrate that CAMB is nontoxic to macrophages at concentrations as high as 2.5 μg/mL, whereas the conventional formulation, AmB deoxycholate, showed high toxicity at this concentration. The in vitro activity of CAMB against L. chagasi was found to be similar to that of the reference drug AmB deoxycholate, with ED50s of 0.017 μg/mL and 0.021 μg/mL, respectively. Considering that L. chagasi affects organs amenable to cochleate-mediated delivery of AmB, we hypothesize that CAMB will be an effective lipid system for the treatment of visceral leishmaniasis.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Amphotericin B
/
Macrophages, Peritoneal
/
Leishmania infantum
/
Antiprotozoal Agents
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Espírito Santo/BR