In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
Biocell
; 34(2): 71-79, Aug. 2010. ilus, graf
Article
in English
| LILACS
| ID: lil-595041
Responsible library:
AR40.1
ABSTRACT
In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM's in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4 microM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased sub-G1 and G2/M fractions, as well as an increase in cycli n B1 levels were observed. Further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Cell Cycle
/
Cell Line, Tumor
/
Cell Proliferation
/
Estriol
Type of study:
Prognostic study
Limits:
Female
/
Humans
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2010
Document type:
Article
Affiliation country:
South Africa
Institution/Affiliation country:
University of Pretoria/ZA