Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro / Identification of Mycobacterium bovis among mycobacterial isolates from human clinical specimens at a university hospital in Rio de Janeiro, Brazil
J. bras. pneumol
; 37(5): 664-668, set.-out. 2011. tab
Article
in Portuguese
| LILACS
| ID: lil-604395
Responsible library:
BR1.1
RESUMO
Entre 2005 e 2006, 8.121 espécimes clínicos enviados ao Laboratório de Micobactérias do Hospital Universitário Clementino Fraga Filho/Instituto de Doenças do Tórax, no Rio de Janeiro, RJ, foram inoculados em meio Löwenstein-Jensen contendo glicerol e piruvato. Desses espécimes, 79 isolados de micobactérias tiveram crescimento somente em meio com piruvato, sendo selecionados para a identificação presuntiva de Mycobacterium bovis. Esses isolados foram submetidos à identificação por testes bioquímicos, amplificação por PCR com primers específicos (Rv0577 e Rv1510) e teste de suscetibilidade à pirazinamida. Todas as cepas apresentaram padrões fenotípicos e genotípicos de M. tuberculosis, não sendo detectado M. bovis.
ABSTRACT
In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were screened with biochemical tests, PCR amplification (with the specific primers Rv0577 and Rv1510), and pyrazinamide susceptibility tests. All of the strains isolated showed specific phenotypical and genotypical patterns characteristic of M. tuberculosis, and no M. bovis strains were detected.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Tuberculosis
/
Zoonoses
Database:
LILACS
Main subject:
Tuberculosis
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
Portuguese
Journal:
J. bras. pneumol
Journal subject:
Pulmonary Disease (Specialty)
Year:
2011
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal Fluminense/BR
/
Universidade Federal do Rio de Janeiro/BR