Histomorphometric assessment of bone necrosis produced by two cryosurgery protocols using liquid nitrogen: an experimental study on rat femurs
J. appl. oral sci
; 19(6): 604-609, Nov.-Dec. 2011. ilus, graf
Article
in English
| LILACS
| ID: lil-610874
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
The aim of this study was to evaluate the effects of liquid nitrogen cryosurgery on the femoral diaphysis of rats. MATERIAL ANDMETHODS:
The femoral diaphyses of 42 Wistar rats were exposed to three local and sequential applications of liquid nitrogen for 1 or 2 min, intercalated with periods of 5 min of passive thawing. The animals were sacrificed after 1, 2, 4 and 12 weeks and the specimens obtained were processed and analyzed histomorphometrically.RESULTS:
The depth and extent of peak bone necrosis were 124.509 µm and 2087.094 µm for the 1-min protocol, respectively, and 436.424 µm and 12046.426 µm for the 2-min protocol. Peak necrosis was observed in the second experimental week with both cryotherapy protocols.CONCLUSIONS:
The present results indicate that the 2-min protocol produced more marked bone necrosis than the 1-min protocol. Although our results cannot be entirely extrapolated to clinical practice, they contribute to the understanding of the behavior of bone tissue submitted to different cycles of liquid nitrogen freezing and may serve as a basis for new studies.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Cryosurgery
/
Femur
/
Femur Head Necrosis
/
Nitrogen
Type of study:
Evaluation study
/
Practice guideline
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2011
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Ceará/BR