Genetic diversity of Leishmania infantum field populations from Brazil
Mem. Inst. Oswaldo Cruz
; 107(1): 39-47, Feb. 2012. ilus, mapas, tab
Article
in English
| LILACS
| ID: lil-612804
Responsible library:
BR1.1
ABSTRACT
Leishmania infantum (syn. Leishmania chagasi) is the etiological agent of visceral leishmaniasis (VL) in Brazil. The epidemiology of VL is poorly understood. Therefore, a more detailed molecular characterization at an intraspecific level is certainly needed. Herein, three independent molecular methods, multilocus microsatellite typing (MLMT), random amplification of polymorphic DNA (RAPD) and simple sequence repeats-polymerase chain reaction (SSR-PCR), were used to evaluate the genetic diversity of 53 L. infantum isolates from five different endemic areas in Brazil. Population structures were inferred by distance-based and Bayesian-based approaches. Eighteen very similar genotypes were detected by MLMT, most of them differed in only one locus and no correlation was found between MLMT profiles, geographical origin or the estimated population structure. However, complex profiles composed of 182 bands obtained by both RAPD and SSR-PCR assays gave different results. Unweighted pair group method with arithmetic mean trees built from these data revealed a high degree of homogeneity within isolates of L. infantum. Interestingly, despite this genetic homogeneity, most of the isolates clustered according to their geographical origin.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Genetic Variation
/
DNA, Protozoan
/
Leishmania infantum
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2012
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Natan Portella para Doenças Tropicais/BR
/
Instituto René Rachou/BR
/
Universidade Estadual de Montes Claros/BR
/
Universidade Federal da Paraíba/BR
/
Universidade Federal de Minas Gerais/BR
/
Universidade Federal do Espírito Santo/BR