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Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
Vaucher, Rodrigo de Almeida; Dezen, Diogenes; Simonetti, Amauri Braga; Spilki, Fernando Rosado; Roehe, Paulo Michel.
Affiliation
  • Vaucher, Rodrigo de Almeida; s.af
  • Dezen, Diogenes; s.af
  • Simonetti, Amauri Braga; Universidade Federal do Rio Grande do Sul. Departamento de Microbiologia. Porto Alegre. BR
  • Spilki, Fernando Rosado; Universidade Feevale. Novo Hamburgo. BR
  • Roehe, Paulo Michel; Universidade Federal do Rio Grande do Sul. Departamento de Microbiologia. Concordia, SC. BR
Braz. j. microbiol ; 42(4): 1440-1444, Oct.-Dec. 2011. ilus, tab
Article in En | LILACS | ID: lil-614608
Responsible library: BR32.1
ABSTRACT
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Respirovirus Infections / Phylogeny / In Vitro Techniques / Base Sequence / Reverse Transcriptase Polymerase Chain Reaction / Reverse Transcription Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2011 Document type: Article Affiliation country: Brazil Country of publication: Brazil
Fulltext
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Respirovirus Infections / Phylogeny / In Vitro Techniques / Base Sequence / Reverse Transcriptase Polymerase Chain Reaction / Reverse Transcription Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2011 Document type: Article Affiliation country: Brazil Country of publication: Brazil