An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
Mem. Inst. Oswaldo Cruz
; 107(2): 194-197, Mar. 2012. ilus
Article
in En
| LILACS
| ID: lil-617064
Responsible library:
BR1.1
ABSTRACT
The aim of this work was to evaluate a dot-enzyme-linked immunosorbent assay (dot-ELISA) using excretory-secretory antigens from the larval stages of Toxocara canis for the diagnosis of toxocariasis. A secondary aim was to establish the optimal conditions for its use in an area with a high prevalence of human T. canis infection. The dot-ELISA test was standardised using different concentrations of the antigen fixed on nitrocellulose paper strips and increasing dilutions of the serum and conjugate. Both the dot-ELISA and standard ELISA methods were tested in parallel with the same batch of sera from controls and from individuals living in the problem area. The best results were obtained with 1.33 µg/mL of antigen, dilutions of 1/80 for the samples and controls and a dilution of 1/5,000 for the anti-human IgG-peroxidase conjugate. All steps of the procedure were performed at room temperature. The coincidence between ELISA and dot-ELISA was 85 percent and the kappa index was 0.72. The dot-ELISA test described here is rapid, easy to perform and does not require expensive equipment. Thus, this test is suitable for the serological diagnosis of human T. canis infection in field surveys and in the primary health care centres of endemic regions.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Toxocariasis
/
Toxocara canis
/
Antigens, Helminth
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Country/Region as subject:
America do sul
/
Argentina
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2012
Document type:
Article
Affiliation country:
Argentina
Country of publication:
Brazil