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Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
Maluta, Renato Pariz; Stella, Ariel Eurides; Riccardi, Kátia; Rigobelo, Everlon Cid; Marin, José Moacir; Carvalho, Marileda Bonafim; Ávila, Fernando Antonio de.
Affiliation
  • Maluta, Renato Pariz; Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal. BR
  • Stella, Ariel Eurides; Universidade Federal de Goiás. Jataí. BR
  • Riccardi, Kátia; Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal. BR
  • Rigobelo, Everlon Cid; Universidade Estadual Paulista. Dracena. BR
  • Marin, José Moacir; Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Ribeirão Preto. BR
  • Carvalho, Marileda Bonafim; Universidade Estadual Paulista. Faculdade de Ciências Agrárias. Jaboticabal. BR
  • Ávila, Fernando Antonio de; Universidade Estadual Paulista. Faculdade de Ciências Agrárias. Jaboticabal. BR
Braz. j. microbiol ; 43(1): 375-381, Jan.-Mar. 2012. ilus, tab
Article in English | LILACS | ID: lil-622827
Responsible library: BR32.1
ABSTRACT
Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E.
Subject(s)


Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Zoonoses Database: LILACS Main subject: Urinary Tract / In Vitro Techniques / Drug Resistance, Microbial / Polymerase Chain Reaction / Bacterial Typing Techniques / Escherichia coli / Escherichia coli Infections / Biomarkers, Pharmacological Type of study: Diagnostic study / Evaluation study / Prognostic study Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2012 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Goiás/BR / Universidade de São Paulo/BR

Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Zoonoses Database: LILACS Main subject: Urinary Tract / In Vitro Techniques / Drug Resistance, Microbial / Polymerase Chain Reaction / Bacterial Typing Techniques / Escherichia coli / Escherichia coli Infections / Biomarkers, Pharmacological Type of study: Diagnostic study / Evaluation study / Prognostic study Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2012 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Goiás/BR / Universidade de São Paulo/BR
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