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Microwave Fixation in Rat Fetuses Tissues: Histological and Immunohistochemical Analysis / Fijación de tejidos de Fetos de Ratas por Microondas: Análisis Histológico e Inmunohistoquímico
Pitol, Dimitrius Leonardo; Mardegan Issa, João Paulo Mardegan; Camacho, Eduardo; Wolga, Nilce de Oliveira; Caetano, Flávio Henrique; Lunardi, Laurelúcia Orive.
Affiliation
  • Pitol, Dimitrius Leonardo; UNESP. Biosciences Institute- Molecular and Cellular Biology. Rio Claro. BR
  • Mardegan Issa, João Paulo Mardegan; University of São Paulo. Faculty of Dentistry of Ribeirão Preto. BR
  • Camacho, Eduardo; UNESP. Biosciences Institute- Molecular and Cellular Biology. Rio Claro. BR
  • Wolga, Nilce de Oliveira; University of São Paulo. Faculty of Dentistry of Ribeirão Preto. BR
  • Caetano, Flávio Henrique; UNESP. Biosciences Institute- Molecular and Cellular Biology. Rio Claro. BR
  • Lunardi, Laurelúcia Orive; University of São Paulo. Faculty of Dentistry of Ribeirão Preto. BR
Int. j. morphol ; 25(4): 695-701, Dec. 2007. ilus
Article in English | LILACS | ID: lil-626925
Responsible library: CL1.1
ABSTRACT
The aim of this study was to show the microwaves action in fixation of rat fetuses, dermal and cartilaginous tissues, using histological and immunohistochemistry methods for analysis. It was possible to conclude in this study using the rat as experimental model that the two methods for antibody retrieval, presented an excellent ways for the use of Ki67 antibody in the immunohistochemical analysis.
RESUMEN
El objetivo de este estudio fue evaluar la acción de las microondas en la fijación de los tejidos dérmico y cartilaginoso de fetos de ratas, usando para el análisis métodos histológico e inmunohistoquímico. Fue posible concluir en este estudio usando la rata como modelo experimental, que los dos métodos empleados para la recuperación antigénica representan excelentes medios para el uso del anticuerpo Ki67, en el análisis inmunohistoquímico.
Subject(s)

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Full text: Available Collection: International databases Database: LILACS Main subject: Tissue Fixation / Fetus / Microwaves Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2007 Document type: Article Affiliation country: Brazil Institution/Affiliation country: UNESP/BR / University of São Paulo/BR
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Full text: Available Collection: International databases Database: LILACS Main subject: Tissue Fixation / Fetus / Microwaves Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2007 Document type: Article Affiliation country: Brazil Institution/Affiliation country: UNESP/BR / University of São Paulo/BR