Polimorfismos de receptores semejantes a Toll 2 y 4 en pacientes con asma bronquial y enfermedad pulmonar obstructiva crónica (EPOC) / Polymorphisms of Toll-like receptors 2 and 4 in patients with bronchial asthma and chronic obstructive pulmonary disease (COPD)
Rev. Fac. Med. (Caracas)
; 31(2): 116-122, dic. 2008. tab
Article
in Spanish
| LILACS
| ID: lil-631528
Responsible library:
VE1.1
RESUMEN
Los receptores semejantes a Toll forman parte del sistema inmune. Algunas mutaciones en estos receptores se han relacionado con el desarrollo de enfermedades como el asma bronquial y la enfermedad pulmonar obstructiva crónica. Se realizó un estudio de casos-controles, en el que se incluyeron 100 pacientes con asma bronquial, 100 pacientes con enfermedad pulmonar obstructiva crónica y 100 controles. A través de la técnica de reacción en cadena de la polimerasa, se investigó la presencia de polimorfismos en TLR2 (Arg677Trp y Arg753Gln) y TLR4 (Asp299Gly y Thr399Ile) y su asociación con asma y enfermedad pulmonar obstructiva crónica. Se demostró la presencia de polimorfismos en TLR4 con una frecuencia del 9 por ciento en cada grupo de estudio. No se observó relación alguna entre la presencia de polimorfismos en TLR4 y los índices de gravedad para asma bronquial o enfermedad pulmonar obstructiva crónica. La gravedad de los pacientes con asma y enfermedad pulmonar obstructiva crónica no está relacionada con los polimorfismos en TLR 4
ABSTRACT
Toll like receptors are important in the inflammatory immune response. Several mutations of these receptors have been related to the development and chronicity of different inflammatory diseases as bronchial asthma and obstructive pulmonary disease. A case control study was performed, 100 patients with bronchial asthma, 100 obstructive pulmonary disease and 100 controls. Restriction based polymorphism PCR analysis was performed for TLR2 (Arg677Trp and Arg753Gln) and TLR4 (Asp299Gly and Thr399Ile) genes and its association with asthma and obstructive pulmonary disease was assessed. The frequency of TLR4 polymorphism was 9 percent in each group studied (asthma, obstructive pulmonary disease and controls). No association was found among TLR4 polymorphism and stage of disease for asthma or obstructive pulmonary disease. TLR4 polymorphism are not associated with the presence or severity of asthma or obstructive pulmonary disease in the Venezuelan population
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Asthma
/
Pulmonary Disease, Chronic Obstructive
/
Amplified Fragment Length Polymorphism Analysis
Type of study:
Evaluation study
/
Observational study
/
Risk factors
Limits:
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. Fac. Med. (Caracas)
Journal subject:
Medicine
Year:
2008
Document type:
Article
Affiliation country:
Venezuela
Institution/Affiliation country:
Hospital Universitario de Caracas/VE
/
Universidad Central de Venezuela/VE