Methicillin-resistant Staphylococcus aureus strains in Buenos Aires Teaching Hospitals: replacement of the multidrug resistant South American clone by another susceptible to rifampin, minocycline and trimethoprim-sulfamethoxazole / Staphylococcus aureus meticilina-resistente en hospitales universitarios de Buenos Aires: reemplazo del clon Sudamericano multi-resistente por otro sensible a rifampicina, minociclina y trimetoprima-sulfametoxazol
Rev. argent. microbiol
; 37(3): 156-160, jul.-sep. 2005. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-634498
Responsible library:
AR1.2
ABSTRACT
The aim of this study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from different infectious sites of hospitalized patients at two university hospitals. Fourteen isolates were analyzed by repetitive sequence based PCR (Rep-PCR), randomly amplified polymorphic DNA assay (RAPD-PCR), and pulsed-field gel electrophoresis (PFGE). We found that a prevalent clone of MRSA, susceptible to rifampin, minocycline, and trimethoprim/sulfamethoxazole (RIF S, MIN S, TMS S) was present in both hospitals in replacement of the multiresistant MRSA South American clone, previously described in these hospitals. The staphylococcal chromosomal cassette (SCCmec) type I element was detected in this new clone.
RESUMEN
El objetivo de este trabajo fue la caracterización de aislamientos de Staphylococcus aureus meticilina-resistentes (SAMR), provenientes de diferentes procesos infecciosos de pacientes internados en dos hospitales universitarios. Catorce aislamientos fueron analizados mediante la PCR de secuencias repetitivas (Rep-PCR), la amplificación al azar de ADN polimórfico (RAPD-PCR) y la electroforesis de campo pulsado (PFGE). Encontramos que un clon prevalente de SAMR, sensible a rifampicina, minociclina y trimetoprima-sulfametoxazol (RIF S, MIN S, TMS S) estaba presente en ambos hospitales, reemplazando al clon SAMR y multi-resistente previamente descrito en estos mismos hospitales. En este nuevo clon se detectó el cassette cromosómico estafilocócico SCCmec tipo I.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Staphylococcal Infections
/
Staphylococcus aureus
/
Cross Infection
/
Methicillin Resistance
/
Drug Resistance, Multiple, Bacterial
/
Hospitals, University
Type of study:
Controlled clinical trial
Limits:
Humans
Country/Region as subject:
South America
/
Argentina
Language:
Spanish
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2005
Document type:
Article
/
Project document
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad de Buenos Aires/AR
/
Universidad de Buenos Aires/BR