Diferenciación del complejo Entamoeba histolytica/Entamoeba dispar mediante Gal/GalNAc-lectina y PCR en aislamientos colombianos / Differentiation of entamoeba histolytica from entamoeba dispar using Gal/GalNAc-lectin and polymerase chain reaction
Rev. méd. Chile
; 140(4): 476-483, abr. 2012. ilus
Article
in Spanish
| LILACS
| ID: lil-643217
Responsible library:
CL1.1
ABSTRACT
Background:
Entamoeba histolytica and Entamoeba dispar are morphologically identical. However, the former is highly pathogenic and the latter is not.Aim:
To differentiate Entamoeba histolytica from Entamoeba dispar through ELISA and PCR techniques in Colombian isolates from feces. Material andMethods:
Descriptive study of Colombian fecal samples from 53 males and 47 women, that were positive for the complex E. histolytica/E. dispar on light microscopy. Positive samples were cultured on Robinson medium to isolate trophozoites. The presence of specific Gal/ GalNAc-lectin was determined by ELISA and polymerase chain reaction in genomic DNA, using the combination of three nucleotides that recognize a variable region of 16S small subunit ribosomal RNA, generating a 166 base pair (bp) product for E. histolytica and 752 pb product for E. dispar.Results:
After verification, only eight of the 100 samples were positive for the complex E. histolytica/E. dispar and were cultivated. Isolates were obtained in six cultures, one corresponded to E. histolytica and six to E. dispar.Conclusions:
The presence of E. histolytica/E. dispar complex was largely overestimated with light microscopy. In the few samples where isolates were obtained, the technique described differentiated between both strains.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Zoonoses
Database:
LILACS
Main subject:
Entamoeba
/
Entamoebiasis
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Colombia
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2012
Document type:
Article
/
Project document
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad Nacional de Colombia/CO