Isolation of the first IMP-4 metallo beta lactamase producing Klebsiella pneumoniae in Tianjin, China
Braz. j. microbiol
; 43(3): 917-922, July-Sept. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-656653
Responsible library:
BR32.1
ABSTRACT
This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin medical university general hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. ClassⅠintegron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in ClassⅠintegron 5'CS. The gene determinants were organized in the order of blaIMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
LILACS
Main subject:
In Vitro Techniques
/
Drug Resistance, Microbial
/
Base Sequence
/
Carbapenems
/
Polymerase Chain Reaction
/
Integrons
/
Metalloproteases
/
Disease Susceptibility
/
Klebsiella pneumoniae
Limits:
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2012
Document type:
Article
Affiliation country:
China
Institution/Affiliation country:
Tianjin Medical University General Hospital/CN