Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital
Braz. j. infect. dis
; 16(6): 521-526, Nov.-Dec. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-658921
Responsible library:
BR1.1
ABSTRACT
INTRODUCTION:
Acinetobacter spp. have emerged as notorious pathogens involved in healthcareassociated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Acinetobacter spp. Different mechanisms may confer resistance to these drugs in the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out as one of the predominant carbapenamases among Acinetobacter. The present work aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacter isolates recovered from patients of a university hospital in Niterói, RJ, Brazil.METHODS:
Antimicrobial susceptibility profiles were determined by disk-diffusion. Imipenem resistant isolates were submitted to Modified Hodge Test in order to screen for carbapenemase production, and later to polymerase chain reaction (PCR) to investigate the presence of blaOXA-23.RESULTS:
Imipenem and meropenem resistance rates were 71.4% and 69.7%, respectively. The Modified Hodge Test revealed carbapenemase production among 76 (89.4%) of the 85 imipenem resistant isolates analyzed; according to PCR results, 81 isolates (95.4%) carried the blaOXA-23 gene.CONCLUSIONS:
OXA-23-like enzymes may be an important mechanism of carbapenem resistance among isolates present in the hospital studied.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Acinetobacter
/
Beta-Lactamases
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2012
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal Fluminense/BR