Conventional serological performance in diagnosis of Chagas' disease in southern Brazil
Braz. j. infect. dis
; 17(2): 174-178, Mar.-Apr. 2013. tab
Article
in English
| LILACS
| ID: lil-673196
Responsible library:
BR1.1
ABSTRACT
Results of Chagas' disease diagnosis show disagreement. The aim of this study was to compare commercial tests for Chagas' disease serodiagnosis in southern Brazil. A total of 161 samples were evaluated. Three enzyme-linked immunosorbent assays, one indirect hemagglutination and one indirect immunofluorescence were assessed. Trypomastigote excreted-secreted antigen-blot was a confirmatory method. From 161 samples, 65.84% were positive in all tests, while 34.16% presents mismatch result in at least one of the tests. All techniques tested presented false-positive and/or false-negative results as follows Enzyme-linked immunosorbent assay 1 had more false-positive results (lower specificity), indirect immunofluorescence had the highest rate of false-negative results (lower sen sitivity), enzyme-linked immunosorbent assays had fewer false-negative results (higher sensitivity), while indirect hemagglutination showed no false-positive result (higher specificity). Knowing the characteristics of techniques make it possible to combine them and obtain more reliable diagnosis. Therefore, it seems useful to combine techniques for diagnosing this infection.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Chagas Disease
/
Neglected Diseases
Database:
LILACS
Main subject:
Trypanosoma cruzi
/
Antibodies, Protozoan
/
Chagas Disease
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2013
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Pelotas/BR