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Determination of cellular debris in endodontically treated teeth: a pilot study / Determinación de restos celulares en dientes endodonciados: estudio piloto
Rivera, César; Muñoz, Camila; Suazo, Iván.
Affiliation
  • Rivera, César; University of Talca. Faculty of Health Sciences. Department of Basic Biomedical Sciences. Talca. CL
  • Muñoz, Camila; University of Talca. Talca. CL
  • Suazo, Iván; Diego Portales University. Faculty of Medicine. Santiago. CL
Int. j. odontostomatol. (Print) ; 6(3): 291-295, 2012. ilus
Article in En | LILACS | ID: lil-676188
Responsible library: CL1.1
ABSTRACT
Previous studies indicate that dental tissues are a source of mitochondrial DNA that could be useful for human identification. The main cell in the pulpo-dentin complex is the odontoblast, whose cellular body is located on the border between the pulp and dentin and continues through cell processes. In endodontically treated teeth, pulp tissue is removed, assuming the complete elimination of cellular content and the inner third of dentin. Facing the possibility of finding teeth that were treated endodontically as the only source available for a forensic analysis, is that the objective of this study is to determine the presence of cellular debris in the dentin of teeth with root canal treatment. Twenty teeth roots obtained from 8 single and multi-rooted teeth were treated endodontically, with conventional manual technique. The samples were processed by conventional histological analysis (H&E). In root canals endodontic cement remnants and cylinder-cubic structures resembled odontoblastic bodies were observed, but without certainty to establish its presence. This research concludes that it is not possible to determine presence of cellular debris in endodontically treated teeth using the described technique...
RESUMEN
Estudios previos indican que los tejidos dentales son fuente de DNA mitocondrial útiles para la identificación humana. La principal célula del complejo pulpo-dentinario es el odontoblasto, cuyo cuerpo celular ubicado en el límite entre la pulpa y la dentina se continúa por prolongaciones celulares. En dientes tratados endodónticamente se extrae el tejido pulpar, presumiendo la completa eliminación del contenido celular y el tercio interno de la dentina. Frente a la posibilidad de encontrar dientes que fueron tratados endodónticamente como única fuente disponible para análisis forense, es que el objetivo de este estudio es determinar la presencia de restos celulares en la dentina de dientes con tratamiento de canales radiculares. 20 raíces dentarias obtenidas de 8 dientes uni y multirradiculares, fueron tratadas endodónticamente con terapia manual convencional. Las muestras fueron procesadas mediante análisis histológico convencional (H&E). En los canales radiculares se observaron restos de cemento endodóntico y estructuras cilindro-cúbicas que asemejaron a cuerpos de odontoblastos, sin poder establecer con certeza su presencia. En esta investigación se concluye que no es posible determinar mediante la técnica utilizada la presencia de restos celulares en dientes tratados endodónticamente...
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Root Canal Therapy / DNA, Mitochondrial / Tooth, Nonvital / Odontoblasts Type of study: Guideline / Prognostic_studies Limits: Humans Language: En Journal: Int. j. odontostomatol. (Print) Journal subject: ODONTOLOGIA Year: 2012 Document type: Article Affiliation country: Chile Country of publication: Chile

Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Root Canal Therapy / DNA, Mitochondrial / Tooth, Nonvital / Odontoblasts Type of study: Guideline / Prognostic_studies Limits: Humans Language: En Journal: Int. j. odontostomatol. (Print) Journal subject: ODONTOLOGIA Year: 2012 Document type: Article Affiliation country: Chile Country of publication: Chile