Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
Braz. j. microbiol
; 44(3): 901-904, July-Sept. 2013. tab
Article
in English
| LILACS
| ID: lil-699786
Responsible library:
BR1.1
ABSTRACT
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Veterinary Medicine
/
Molecular Diagnostic Techniques
/
Penaeidae
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Evaluation study
/
Practice guideline
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2013
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Minas Gerais/BR