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Evaluation of polymerase chain reaction (PCR) for identifying Mycobacterium bovis isolates from the modified Middlebrook 7H11 agar thin layer technique
Rosário, Tatiana Reis do; Dib, Cristina Corsi; Roxo, Eliana; Pinheiro, Sônia Regina; Vasconcellos, Silvio Arruda; Benites, Nilson Roberti.
Affiliation
  • Rosário, Tatiana Reis do; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Dib, Cristina Corsi; Agência Paulista de Tecnologia dos Agronegócios. Instituto Biológico. São Paulo. BR
  • Roxo, Eliana; Agência Paulista de Tecnologia dos Agronegócios. Instituto Biológico. São Paulo. BR
  • Pinheiro, Sônia Regina; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Vasconcellos, Silvio Arruda; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
  • Benites, Nilson Roberti; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo. BR
Rev. Inst. Adolfo Lutz ; 71(4): 753-756, out.-dez. 2012. tab
Article in English | LILACS, Sec. Est. Saúde SP, SESSP-CTDPROD, Sec. Est. Saúde SP, SESSP-ACVSES, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: lil-706148
Responsible library: BR91.2
ABSTRACT
The associated use of the modified Middlebrook 7H11 agar thin layer technique and the Polymerase Chain Reaction (PCR) assay enabled to perform the early identification of microcolonies of Mycobacterium bovis from 12th to 25th day of culture. In order to reduce the time for performing the Mycobacterium bovis identification, the combined use of these two techniques was evaluated by analyzing the microcolonies of mycobacteria at the 8th day after culturing. Until the last day of analysis, all of the PCR-positive samples already showed the microcolonies. Therefore, the early diagnosis of bovine tuberculosis is feasible, without an apparent macroscopic colonies growth.
Subject(s)

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Full text: Available Collection: National databases / Brazil Health context: SDG3 - Health and Well-Being / Neglected Diseases Health problem: Target 3.3: End transmission of communicable diseases / Neglected Diseases / Tuberculosis / Zoonoses Database: LILACS / Sec. Est. Saúde SP / SESSP-ACVSES / SESSP-CTDPROD / SESSP-IALACERVO / SESSP-IALPROD Main subject: Tuberculosis / Polymerase Chain Reaction / Diagnostic Techniques and Procedures / Agar / Mycobacterium bovis Type of study: Diagnostic study / Screening study Language: English Journal: Rev. Inst. Adolfo Lutz Year: 2012 Document type: Article Institution/Affiliation country: Agência Paulista de Tecnologia dos Agronegócios/BR / Universidade de São Paulo/BR
Fulltext
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Full text: Available Collection: National databases / Brazil Health context: SDG3 - Health and Well-Being / Neglected Diseases Health problem: Target 3.3: End transmission of communicable diseases / Neglected Diseases / Tuberculosis / Zoonoses Database: LILACS / Sec. Est. Saúde SP / SESSP-ACVSES / SESSP-CTDPROD / SESSP-IALACERVO / SESSP-IALPROD Main subject: Tuberculosis / Polymerase Chain Reaction / Diagnostic Techniques and Procedures / Agar / Mycobacterium bovis Type of study: Diagnostic study / Screening study Language: English Journal: Rev. Inst. Adolfo Lutz Year: 2012 Document type: Article Institution/Affiliation country: Agência Paulista de Tecnologia dos Agronegócios/BR / Universidade de São Paulo/BR