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Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Rosário, Tatiana Reis do; Dib, Cristina Corsi; Roxo, Eliana; Pinheiro, Sônia Regina; Vasconcellos, Silvio Arruda; Benites, Nilson Roberti.
Affiliation
  • Rosário, Tatiana Reis do; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Dib, Cristina Corsi; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Roxo, Eliana; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Pinheiro, Sônia Regina; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Vasconcellos, Silvio Arruda; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Benites, Nilson Roberti; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
Braz. j. microbiol ; 45(1): 225-230, 2014. ilus, tab
Article in English | LILACS | ID: lil-709456
Responsible library: BR1.1
ABSTRACT
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
Subject(s)


Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Zoonoses Database: LILACS Main subject: Tuberculosis, Bovine / Polymerase Chain Reaction / Bacteriological Techniques / Molecular Diagnostic Techniques / Meat / Mycobacterium bovis Type of study: Diagnostic study / Evaluation study / Prognostic study / Risk factors Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Zoonoses Database: LILACS Main subject: Tuberculosis, Bovine / Polymerase Chain Reaction / Bacteriological Techniques / Molecular Diagnostic Techniques / Meat / Mycobacterium bovis Type of study: Diagnostic study / Evaluation study / Prognostic study / Risk factors Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR
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