Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Braz. j. microbiol
; 45(1): 225-230, 2014. ilus, tab
Article
in English
| LILACS
| ID: lil-709456
Responsible library:
BR1.1
ABSTRACT
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
LILACS
Main subject:
Tuberculosis, Bovine
/
Polymerase Chain Reaction
/
Bacteriological Techniques
/
Molecular Diagnostic Techniques
/
Meat
/
Mycobacterium bovis
Type of study:
Diagnostic study
/
Evaluation study
/
Prognostic study
/
Risk factors
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2014
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR