Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
Acta cir. bras
; 29(8): 485-492, 08/2014. graf
Article
in English
| LILACS
| ID: lil-719186
Responsible library:
BR1.1
ABSTRACT
PURPOSE:
To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS:
This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS:
Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION:
The light-emitting diode is a promising tool for decellularization of soft tissues. .
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Trachea
/
Tissue Engineering
/
Tissue Scaffolds
/
Light
Type of study:
Evaluation study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2014
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Paulista State University/BR