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Development of experimental in vitro burn model
Fernandes, Ana Carolina Morais; França, Jerônimo Pereira de; Gaiba, Silvana; Aloise, Antonio Carlos; Oliveira, Andrea Fernandes de; Moraes, Andrea Aparecida de Fátima Souza; França, Lucimar Pereira de; Ferreira, Lydia Masako.
Affiliation
  • Fernandes, Ana Carolina Morais; Universidade Federal de São Paulo. São Paulo. BR
  • França, Jerônimo Pereira de; Universidade Federal de São Paulo. São Paulo. BR
  • Gaiba, Silvana; Universidade Federal de São Paulo. São Paulo. BR
  • Aloise, Antonio Carlos; Universidade Federal de São Paulo. São Paulo. BR
  • Oliveira, Andrea Fernandes de; Universidade Federal de São Paulo. São Paulo. BR
  • Moraes, Andrea Aparecida de Fátima Souza; Universidade Federal de São Paulo. São Paulo. BR
  • França, Lucimar Pereira de; Universidade Federal de São Paulo. São Paulo. BR
  • Ferreira, Lydia Masako; Universidade Federal de São Paulo. São Paulo. BR
Acta cir. bras ; 29(supl.2): 15-20, 2014. graf
Article in English | LILACS | ID: lil-721379
Responsible library: BR1.1
ABSTRACT

PURPOSE:

To propose an experimental burn model in NIH-3T3 cell line.

METHODS:

Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.

RESULTS:

The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.

CONCLUSION:

The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death. .
Subject(s)

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Full text: Available Collection: International databases Database: LILACS Main subject: Burns / Cell Culture Techniques / Disease Models, Animal Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2014 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Paulo/BR
Fulltext
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Full text: Available Collection: International databases Database: LILACS Main subject: Burns / Cell Culture Techniques / Disease Models, Animal Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2014 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Paulo/BR