Inhibition of acetylcholinesterase activity by hidroalcoholic extractand their fractions of Bouvardia ternifolia (Cav. ) Shcltdl (Rubiaceae) / Inhibición de la actividad de acetilcolinesterasa por el extracto hidroalcohólico y sus fracciones de Bouvardia ternifolia (Cav. ) Shcltdl (Rubiaceae)
Bol. latinoam. Caribe plantas med. aromát
; 11(6): 526-541, nov. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-723583
Responsible library:
CL1.1
ABSTRACT
The hydroalcoholic extract (BtHA) and its fractions of Bouvardia ternifolia were evaluated as inhibitors of the activity of the acetylcholinesterase enzyme utilizing the in vitro method (Ellman). BtHA inhibited the acetylcholinesterase enzyme competitively (IC50 = 0.6 ug/ml); the ethyl acetate fraction (BtF-AcOEt) caused mixed-type inhibition (IC50 = 0.96 ug/ml). A fraction insoluble on methanol (Bt-Faq-1) showed a mixed-type inhibition (CI50 = 0.96 ug/ml). Finally, the methanol-soluble fraction (Bt-Faq-2), presented complex, mixed-type inhibition that corresponds to the C5 system ( alpha= 0.740 and beta = 0.842). Rutin, quercetin, kaempferol and ursolic acid were detected by HPLC and the concentration of these compounds was different in each fraction.
RESUMEN
El extracto hidroalcohólico (BtHA) y fracciones provenientes de Bouvardia ternifolia fueron evaluadas como inhibidores de la enzima acetilcolinesterasa utilizando el método enzimático propuesto por Ellman. BtHA inhibe a la enzima de manera competitiva (IC50 = 0.6 ug/ml); la fracción de acetato de etilo (BtF-AcOEt) provoca una inhibición mixta (IC50 = 0.96 ug/ml). La fracción insoluble en metanol (Bt-Faq-1) mostró una inhibición tipo mixta (CI50 = 0.96 ug/ml). Finalmente la fracción soluble en metanol, Bt-Faq-2, inhibe a la enzima presentando una inhibición mixta que corresponde a un sistema C5 ( alfa= 0.740 and beta= 0.842). Mediante HPLC se detectó rutina, quercetina, canferol y ácido ursólico la concentración de estos compuestos fue diferente en cada fracción.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Acetylcholinesterase
/
Plant Extracts
/
Cholinesterase Inhibitors
/
Rubiaceae
Limits:
Animals
Language:
English
Journal:
Bol. latinoam. Caribe plantas med. aromát
Journal subject:
Botany
/
Medicine
/
Plantas Medicinais
/
Terapias Complementares
Year:
2012
Document type:
Article
/
Project document
Affiliation country:
Mexico
Institution/Affiliation country:
Instituto Mexicano del Seguro Social/MX
/
Instituto Politécnico Nacional/MX