Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
Braz. j. microbiol
; Braz. j. microbiol;45(3): 841-843, July-Sept. 2014. ilus
Article
in En
| LILACS
| ID: lil-727011
Responsible library:
BR1.1
ABSTRACT
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Tuberculosis
/
Bacteriological Techniques
/
Molecular Diagnostic Techniques
/
Multiplex Polymerase Chain Reaction
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Type of study:
Diagnostic_studies
/
Evaluation_studies
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2014
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil