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Genetic characterization of Toxoplasma gondii isolates from eared doves (Zenaida auriculata) in Brazil / Caracterização genética de isolados de Toxoplasma gondii de pombos (Zenaida auriculata) no Brasil
Barros, Luiz Daniel de; Taroda, Alessandra; Zulpo, Dauton Luiz; Cunha, Ivo Alexandre Leme da; Sammi, Ana Sue; Cardim, Sérgio Tosi; Miura, Ana Carolina; Su, Chunlei; Machado, Rosangela Zacarias; Vidotto, Odilon; Garcia, João Luis.
Affiliation
  • Barros, Luiz Daniel de; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Taroda, Alessandra; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Zulpo, Dauton Luiz; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Cunha, Ivo Alexandre Leme da; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Sammi, Ana Sue; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Cardim, Sérgio Tosi; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Miura, Ana Carolina; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Su, Chunlei; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Machado, Rosangela Zacarias; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Vidotto, Odilon; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
  • Garcia, João Luis; Universidade Estadual de Londrina – UEL. Department of Preventive Veterinary Medicine. Protozoology Laboratory. Londrina. BR
Rev. bras. parasitol. vet ; 23(4): 443-448, Oct-Dec/2014. tab
Article in English | LILACS | ID: lil-731244
Responsible library: BR1.1
ABSTRACT
Eared doves (Zenaida auriculata), which are common in urban, rural and wild areas in many regions of Brazil, are frequently prey for domestic cats. Therefore Toxoplasma gondii isolates obtained from doves may reflect greater environmental diversity than those from other hosts. The aim of the present study was to evaluate T. gondii seroprevalence, isolate and genotype strains from Z. auriculata. Serum and tissue samples were collected from 206 doves for use in the modified agglutination test (MAT) and mouse bioassay. The prevalence of T. gondii antibodies in the doves was 22.3% (46/206), with titers ranging from 16 to 4096, and T. gondii strains were isolated from 12 of these doves. Five genotypes were detected by means of PCR-RFLP, including ToxoDB genotypes #1, #6, #17 and #65, and one genotype that had not previously been described (ToxoDB#182). This was the first report on isolation of T. gondii from Z. auriculata. This study confirmed the genetic diversity of T. gondii isolates and the existence of clonal type II (ToxoDB genotype #1) in Brazil.
RESUMO
Pombos silvestres (Zenaida auriculata), comuns em áreas urbanas, rurais e selvagens em muitas regiões do Brasil, são frequentemente predados por gatos domésticos. Sendo assim, os isolados de T. gondii obtidos de pombos podem refletir uma maior diversidade ambiental do que os outros hospedeiros. O objetivo do presente estudo foi avaliar a soroprevalência, isolar e genotipar T. gondii de Z. auriculata. Amostras de soro e tecido foram coletadas de 206 pombos para o teste de aglutinação modificado (MAT) e o bioensaio em camundongos. A prevalência de anticorpos contra T. gondii em pombos foi 22,3% (46/206), com títulos variando de 16 a 4096, e T. gondii foi isolado de 12 pombos. Cinco genótipos foram detectados por PCR-RFLP, incluindo os genótipos ToxoDB #1, #6, #17, #65 e um genótipo não descrito anteriormente (ToxoDB#182). Esse é o primeiro relato de isolamento de T. gondii de Z. auriculata. Este estudo também confirmou a diversidade dos isolados de T. gondii e a presença de tipo clonal II (ToxoDB #1) no Brasil.
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Full text: Available Collection: International databases Database: LILACS Main subject: Phagosomes / Iron / Macrophages / Nitric Oxide Type of study: Risk factors Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Rev. bras. parasitol. vet Journal subject: Veterinary Medicine / Parasitology Year: 2014 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual de Londrina – UEL/BR
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Full text: Available Collection: International databases Database: LILACS Main subject: Phagosomes / Iron / Macrophages / Nitric Oxide Type of study: Risk factors Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Rev. bras. parasitol. vet Journal subject: Veterinary Medicine / Parasitology Year: 2014 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual de Londrina – UEL/BR