Long-term, regular remote ischemic preconditioning improves endothelial function in patients with coronary heart disease
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;48(6): 568-576, 06/2015. tab, graf
Article
in En
| LILACS
| ID: lil-748228
Responsible library:
BR1.1
ABSTRACT
Remote ischemic preconditioning (RIPre) can prevent myocardial injury. The purpose of this study was to assess the beneficial effects of long-term regular RIPre on human arteries. Forty patients scheduled for coronary artery bypass graft (CABG) surgery were assigned randomly to a RIPre group (n=20) or coronary heart disease (CHD) group (n=20). Twenty patients scheduled for mastectomy were enrolled as a control group. RIPre was achieved by occluding arterial blood flow 5 min with a mercury sphygmomanometer followed by a 5-min reperfusion period, and this was repeated 4 times. The RIPre procedure was repeated 3 times a day for 20 days. In all patients, arterial fragments discarded during surgery were collected to evaluate endothelial function by flow-mediated dilation (FMD), CD34+ monocyte count, and endothelial nitric oxide synthase (eNOS expression). Phosphorylation levels of STAT-3 and Akt were also assayed to explore the underlying mechanisms. Compared with the CHD group, long-term regular RIPre significantly improved FMD after 20 days (8.5±2.4 vs 4.9±4.2%, P<0.05) and significantly reduced troponin after CABG surgery (0.72±0.31 and 1.64±0.19, P<0.05). RIPre activated STAT-3 and increased CD34+ endothelial progenitor cell counts found in arteries. Long-term, regular RIPre improved endothelial function in patients with CHD, possibly due to STAT-3 activation, and this may have led to an increase in endothelial progenitor cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Endothelium, Vascular
/
Ischemic Preconditioning, Myocardial
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Coronary Disease
Type of study:
Clinical_trials
Limits:
Aged
/
Female
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Humans
/
Male
Language:
En
Journal:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
2015
Document type:
Article
Affiliation country:
China
Country of publication:
Brazil