Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
Braz. arch. biol. technol
; Braz. arch. biol. technol;58(6): 923-928, Nov.-Dec. 2015. graf
Article
in En
| LILACS
| ID: lil-766966
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Language:
En
Journal:
Braz. arch. biol. technol
Journal subject:
BIOLOGIA
Year:
2015
Document type:
Article
/
Project document
Affiliation country:
Brazil
Country of publication:
Brazil