Antigens, CD/metabolism; Metalloendopeptidases/genetics; Metalloendopeptidases/physiology; Protein Precursors/antagonists & inhibitors; Protein Precursors/metabolism; Receptors, Tumor Necrosis Factor/metabolism; Tumor Necrosis Factor-alpha/antagonists & inhibitors; Tumor Necrosis Factor-alpha/metabolism; ADAM Proteins; ADAM10 Protein; ADAM17 Protein; Amyloid Precursor Protein Secretases; Antigens, CD/biosynthesis; Antigens, CD/genetics; Cell Line; Cytoplasm/metabolism; Cytoplasm/physiology; DNA, Complementary/genetics; Disintegrins/genetics; Disintegrins/physiology; Enzyme Activation/genetics; Flow Cytometry; Humans; Membrane Proteins/antagonists & inhibitors; Membrane Proteins/biosynthesis; Metalloendopeptidases/antagonists & inhibitors; Metalloendopeptidases/biosynthesis; Metalloendopeptidases/chemistry; Peptide Fragments/genetics; Peptide Fragments/physiology; Protein Precursors/biosynthesis; Protein Precursors/genetics; Protein Processing, Post-Translational/genetics; Protein Processing, Post-Translational/immunology; Receptors, Tumor Necrosis Factor/biosynthesis; Receptors, Tumor Necrosis Factor/genetics; Receptors, Tumor Necrosis Factor, Type II; Solubility; Substrate Specificity/genetics; Transfection; Tumor Necrosis Factor-alpha/biosynthesis; Tumor Necrosis Factor-alpha/genetics