Cystine knot mutations affect the folding of the glycoprotein hormone alpha-subunit. Differential secretion and assembly of partially folded intermediates.

Darling, R J; Ruddon, R W; Perini, F; Bedows, E

Darling, R J; Ruddon, R W; Perini, F; Bedows, E.

Affiliation

Darling RJ; Eppley Institute for Research in Cancer and Allied Diseases and the Department of Pharmacology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

J Biol Chem ; 275(20): 15413-21, 2000 May 19.

Article in En | MEDLINE | ID: mdl-10809777

Subject(s)

Cysteine; Cystine; Glycoprotein Hormones, alpha Subunit/chemistry; Glycoprotein Hormones, alpha Subunit/metabolism; Alanine; Amino Acid Substitution; Cell Line; Disulfides; Dithiothreitol/pharmacology; Humans; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Transfection

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Collection: 01-internacional Database: MEDLINE Main subject: Glycoprotein Hormones, alpha Subunit / Cysteine / Cystine Limits: Humans Language: En Journal: J Biol Chem Year: 2000 Document type: Article Affiliation country: United States Country of publication: United States

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