Identification of a CYP84 family of cytochrome P450-dependent mono-oxygenase genes in Brassica napus and perturbation of their expression for engineering sinapine reduction in the seeds.

Nair, R B; Joy, R W; Kurylo, E; Shi, X; Schnaider, J; Datla, R S; Keller, W A; Selvaraj, G

Nair, R B; Joy, R W; Kurylo, E; Shi, X; Schnaider, J; Datla, R S; Keller, W A; Selvaraj, G.

Affiliation

Nair RB; Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan, Canada, S7N 0W9.

Plant Physiol ; 123(4): 1623-34, 2000 Aug.

Article in En | MEDLINE | ID: mdl-10938378

Subject(s)

Arabidopsis Proteins; Brassica/genetics; Choline/analogs & derivatives; Cytochrome P-450 Enzyme System; Mixed Function Oxygenases/genetics; Seeds/metabolism; Acrolein/analogs & derivatives; Acrolein/metabolism; Amino Acid Sequence; Blotting, Northern; Blotting, Western; Brassica/enzymology; Brassica/metabolism; Choline/metabolism; Chromatography, High Pressure Liquid; Coumaric Acids/metabolism; DNA, Complementary/genetics; Mixed Function Oxygenases/metabolism; Molecular Sequence Data; Open Reading Frames; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Seeds / Brassica / Choline / Cytochrome P-450 Enzyme System / Arabidopsis Proteins / Mixed Function Oxygenases Type of study: Diagnostic_studies Language: En Journal: Plant Physiol Year: 2000 Document type: Article Country of publication: United States

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