High-level autoenhanced expression of a single-copy gene in Escherichia coli: overproduction of bacteriophage T7 protein kinase directed by T7 late genetic elements.

Marchand, I; Nicholson, A W; Dreyfus, M

Marchand, I; Nicholson, A W; Dreyfus, M.

Affiliation

Marchand I; Laboratoire de Génétique Moléculaire (CNRS UMR 8541), ENS, 46 rue d'Ulm, 75230, Paris, France.

Gene ; 262(1-2): 231-8, 2001 Jan 10.

Article in En | MEDLINE | ID: mdl-11179688

Subject(s)

Bacteriophage T7/genetics; Escherichia coli/genetics; Viral Proteins/genetics; Viral Proteins/metabolism; DNA-Directed RNA Polymerases/genetics; Escherichia coli/metabolism; Gene Dosage; Gene Expression Regulation, Bacterial; Point Mutation; Promoter Regions, Genetic; Protein Serine-Threonine Kinases/genetics; Protein Serine-Threonine Kinases/metabolism; RNA Processing, Post-Transcriptional; Transcription, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Viral Proteins / Bacteriophage T7 / Escherichia coli Language: En Journal: Gene Year: 2001 Document type: Article Affiliation country: France Country of publication: Netherlands

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