Mechanism of action of Escherichia coli ribonuclease III. Stringent chemical requirement for the glutamic acid 117 side chain and Mn2+ rescue of the Glu117Asp mutant.

Sun, W; Nicholson, A W

Sun, W; Nicholson, A W.

Affiliation

Sun W; Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA.

Biochemistry ; 40(16): 5102-10, 2001 Apr 24.

Article in En | MEDLINE | ID: mdl-11305928

Subject(s)

Aspartic Acid/chemistry; Endoribonucleases/chemistry; Escherichia coli Proteins; Escherichia coli/enzymology; Glutamic Acid/chemistry; Manganese/chemistry; Mutagenesis, Site-Directed; Aspartic Acid/genetics; Aspartic Acid/metabolism; Base Sequence; Binding Sites/genetics; Catalysis; Catalytic Domain/genetics; Cations, Divalent/chemistry; Endoribonucleases/antagonists & inhibitors; Endoribonucleases/genetics; Endoribonucleases/metabolism; Enzyme Inhibitors/chemistry; Escherichia coli/genetics; Glutamic Acid/genetics; Glutamic Acid/metabolism; Hydrolysis; Magnesium/chemistry; Molecular Sequence Data; Organophosphates/antagonists & inhibitors; Organophosphates/metabolism; Protein Structure, Tertiary/genetics; RNA Processing, Post-Transcriptional/genetics; Ribonuclease III; Substrate Specificity/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Mutagenesis, Site-Directed / Aspartic Acid / Glutamic Acid / Escherichia coli Proteins / Endoribonucleases / Escherichia coli / Manganese Language: En Journal: Biochemistry Year: 2001 Document type: Article Affiliation country: United States Country of publication: United States

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