Construction of a fusion enzyme system by gene splicing as a new molecular recognition element for a sequence biosensor.

Zhou, Y F; Zhang, X E; Liu, H; Zhang, Z P; Zhang, C G; Cass, A E

Zhou, Y F; Zhang, X E; Liu, H; Zhang, Z P; Zhang, C G; Cass, A E.

Affiliation

Zhou YF; Wuhan Institute of Virology, Chinese Academy of Sciences, People's Republic of China.

Bioconjug Chem ; 12(6): 924-31, 2001.

Article in En | MEDLINE | ID: mdl-11716683

Subject(s)

Biosensing Techniques/methods; Enzymes, Immobilized/genetics; Protein Engineering/methods; Recombinant Fusion Proteins/genetics; Artificial Gene Fusion; Aspergillus niger/enzymology; Cloning, Molecular; Enzymes, Immobilized/isolation & purification; Enzymes, Immobilized/metabolism; Glucan 1,4-alpha-Glucosidase/genetics; Glucose Oxidase/genetics; Kinetics; Maltose/analysis; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Reproducibility of Results; Sensitivity and Specificity

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Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Fusion Proteins / Biosensing Techniques / Protein Engineering / Enzymes, Immobilized Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Bioconjug Chem Journal subject: BIOQUIMICA Year: 2001 Document type: Article Country of publication: United States

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