Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis. Gene cloning and protein characterization.

Lönn, Anna; Gárdonyi, Márk; van Zyl, Willem; Hahn-Hägerdal, Bärbel; Otero, Ricardo Cordero

Lönn, Anna; Gárdonyi, Márk; van Zyl, Willem; Hahn-Hägerdal, Bärbel; Otero, Ricardo Cordero.

Affiliation

Lönn A; Department of Applied Microbiology, Lund University, Sweden.

Eur J Biochem ; 269(1): 157-63, 2002 Jan.

Article in En | MEDLINE | ID: mdl-11784309

Subject(s)

Aldose-Ketose Isomerases/genetics; Thermus thermophilus/enzymology; Aldose-Ketose Isomerases/chemistry; Aldose-Ketose Isomerases/metabolism; Cold Temperature; Enzyme Stability; Hydrogen-Ion Concentration; Kinetics; Magnesium/pharmacology; Manganese/pharmacology; Mutagenesis; Polymerase Chain Reaction; Xylitol/pharmacology

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Collection: 01-internacional Database: MEDLINE Main subject: Thermus thermophilus / Aldose-Ketose Isomerases Type of study: Clinical_trials Language: En Journal: Eur J Biochem Year: 2002 Document type: Article Affiliation country: Sweden Country of publication: United kingdom

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