Probing metal ion binding and conformational properties of the colicin E9 endonuclease by electrospray ionization time-of-flight mass spectrometry.

van den Bremer, Ewald T J; Jiskoot, Wim; James, Richard; Moore, Geoffrey R; Kleanthous, Colin; Heck, Albert J R; Maier, Claudia S

van den Bremer, Ewald T J; Jiskoot, Wim; James, Richard; Moore, Geoffrey R; Kleanthous, Colin; Heck, Albert J R; Maier, Claudia S.

Affiliation

van den Bremer ET; Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.

Protein Sci ; 11(7): 1738-52, 2002 Jul.

Article in En | MEDLINE | ID: mdl-12070327

Subject(s)

Bacterial Proteins/metabolism; Colicins; Metals/metabolism; Circular Dichroism; Deoxyribonucleases/metabolism; Ions/metabolism; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrometry, Mass, Electrospray Ionization; Tryptophan/metabolism; Zinc/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Colicins / Metals Language: En Journal: Protein Sci Journal subject: BIOQUIMICA Year: 2002 Document type: Article Affiliation country: Netherlands Country of publication: United States

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