DNA sequencing of close to 1000 bases in 40 minutes by capillary electrophoresis using dimethyl sulfoxide and urea as denaturants in replaceable linear polyacrylamide solutions.

Kotler, Lev; He, Hui; Miller, Arthur W; Karger, Barry L

Kotler, Lev; He, Hui; Miller, Arthur W; Karger, Barry L.

Affiliation

Kotler L; Barnett Institute and Department of Chemistry, Northeastern University, Boston, MA 02115, USA.

Electrophoresis ; 23(17): 3062-70, 2002 Sep.

Article in En | MEDLINE | ID: mdl-12207316

Subject(s)

Electrophoresis, Capillary/methods; Sequence Analysis, DNA/instrumentation; Acrylic Resins; Buffers; DNA/analysis; DNA/chemistry; Dimethyl Sulfoxide; Nucleic Acid Denaturation/drug effects; Reproducibility of Results; Sequence Analysis, DNA/methods; Solutions; Time Factors; Urea

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Collection: 01-internacional Database: MEDLINE Main subject: Sequence Analysis, DNA / Electrophoresis, Capillary Language: En Journal: Electrophoresis Year: 2002 Document type: Article Affiliation country: United States

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