Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis.
Mem Inst Oswaldo Cruz
; 97 Suppl 1: 115-6, 2002.
Article
in En
| MEDLINE
| ID: mdl-12426606
Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Transport Proteins
/
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Carrier Proteins
/
Helminth Proteins
Limits:
Animals
Language:
En
Journal:
Mem Inst Oswaldo Cruz
Year:
2002
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil