Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts.

Gray, Alexander; Olsson, Henric; Batty, Ian H; Priganica, Larisa; Peter Downes, C

Gray, Alexander; Olsson, Henric; Batty, Ian H; Priganica, Larisa; Peter Downes, C.

Affiliation

Gray A; Division of Signal Transduction Therapy, MSI/WTB Complex, University of Dundee, Dow Street, Scotland, Dundee DD1 5EH, UK. a.z.gray@dundee.ac.uk

Anal Biochem ; 313(2): 234-45, 2003 Feb 15.

Article in En | MEDLINE | ID: mdl-12605860

Subject(s)

Phosphatidylinositol 3-Kinases/analysis; Phosphatidylinositols/analysis; Phosphoprotein Phosphatases/analysis; 3T3 Cells; Animals; Binding, Competitive; Biotin/chemistry; Cell Extracts/analysis; Enzyme Inhibitors/pharmacology; Fluorescence Resonance Energy Transfer/methods; HL-60 Cells; Humans; Mice; Oxidative Stress; Phosphatidylinositol 3-Kinases/metabolism; Phosphatidylinositol Phosphates/chemistry; Phosphatidylinositol Phosphates/metabolism; Phosphatidylinositols/isolation & purification; Phosphatidylinositols/metabolism; Phosphoinositide-3 Kinase Inhibitors; Phosphoprotein Phosphatases/metabolism; Phosphoproteins/chemistry; Protein Structure, Tertiary; Recombinant Proteins/analysis; Recombinant Proteins/metabolism; Signal Transduction; Sodium Cholate/pharmacology; Tissue Extracts/analysis; Tissue Extracts/metabolism; Tumor Suppressor Proteins/analysis; Tumor Suppressor Proteins/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Phosphatidylinositols / Phosphoprotein Phosphatases / Phosphatidylinositol 3-Kinases Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Anal Biochem Year: 2003 Document type: Article Affiliation country: United kingdom Country of publication: United States

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