Effect of aeration on lignin peroxidase production by Streptomyces viridosporus T7A.
Appl Biochem Biotechnol
; 105 -108: 799-807, 2003.
Article
in En
| MEDLINE
| ID: mdl-12721417
The effect of aeration on lignin peroxidase production by Streptomyces viridosporus T7A was studied in a bench-scale bioreactor using a previously optimized growth medium (0.65% yeast extract and 0.1% corn oil, pH 7.0) at 37 degrees C and natural pH. Airflow rates of 0.3, 1.0, and 1.5 vvm and a fixed agitation of 200 rpm were initially studied followed by 1.0 vvm and 200, 300, 400, and 500 rpm. The use of 1.0 vvm and 400 rpm increased enzyme concentration 1.8-fold (100-180 U/L) and process productivity 4.8-fold (1.4-6.7 U/[L h]) in comparison with the use of 200 rpm and 0.3 vvm. The inexpensive corn oil, used as carbon source, besides its antifoam properties, proved to be nonrepressive for enzyme production.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peroxidases
/
Streptomyces
/
Biomass
Language:
En
Journal:
Appl Biochem Biotechnol
Year:
2003
Document type:
Article
Affiliation country:
Brazil
Country of publication:
United States