Effect of tryptophan insertions on the properties of the human group IIA phospholipase A2: mutagenesis produces an enzyme with characteristics similar to those of the human group V phospholipase A2.

Beers, Stephen A; Buckland, Andrew G; Giles, Niroshini; Gelb, Michael H; Wilton, David C

Beers, Stephen A; Buckland, Andrew G; Giles, Niroshini; Gelb, Michael H; Wilton, David C.

Affiliation

Beers SA; Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, UK.

Biochemistry ; 42(24): 7326-38, 2003 Jun 24.

Article in En | MEDLINE | ID: mdl-12809488

Subject(s)

Phospholipases A/metabolism; Tryptophan/metabolism; Animals; Catalysis; Cell Membrane/enzymology; Cells, Cultured; Circular Dichroism; Elapidae; Group II Phospholipases A2; Group V Phospholipases A2; Humans; Hydrolysis; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Mutagenesis, Site-Directed; Phosphatidylcholines/chemistry; Phosphatidylcholines/metabolism; Phospholipases A/chemistry; Phospholipases A/genetics; Phospholipases A2; Protein Binding/genetics; Rats; Spectrometry, Fluorescence/methods; Tryptophan/chemistry; Tryptophan/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Phospholipases A / Tryptophan Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biochemistry Year: 2003 Document type: Article Affiliation country: United kingdom Country of publication: United States

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