A new marker on chicken hematopoietic cells is defined by a monoclonal antibody raised against a V beta chain of the human TCR.
Dev Immunol
; 2(4): 273-84, 1992.
Article
in En
| MEDLINE
| ID: mdl-1343096
In this paper, we show that a mouse monoclonal antibody, 111-427, specific for the V beta 5.3 chain of the human T-cell receptor (TCR) for antigen, also reacts with chicken hematopoietic cells. Our data indicate that the majority of 111-427 positive cells among peripheral blood leucocytes (PBL) are thrombocytes. This antibody also recognizes two in vitro cell lines, III-C5, and IL-2-dependent T-cell line and HD11, a macrophage cell line. In addition, erythrocytes and a minor subpopulation of thymus and spleen cells are also stained by the monoclonal antibody (mAb). No specific immunoprecipitation could be detected from 125I radiolabeled cell lysates. By Western blotting techniques, the 111-427 mAb identifies a single band of apparent molecular weight 91 kD, unaffected by reduction, from III-C5 and HD11 cell lysates. This band is absent in negative cell control lysates. On thrombocytes, the apparent molecular weight of the band is shifted to 87 kD. These results indicate that the mAb does not recognize the chicken T-cell receptor for antigen, but a cell surface marker shared primarily between thrombocytes and erythrocytes. This new chicken cell marker is compared to other cell surface markers in avian or mammalian species that present some analogies in their tissue distribution.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Platelets
/
Chickens
/
Receptors, Antigen, T-Cell, alpha-beta
/
Erythrocytes
/
Leukocytes
Limits:
Animals
/
Humans
Language:
En
Journal:
Dev Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
1992
Document type:
Article
Affiliation country:
France
Country of publication:
United kingdom