Mutagenesis of the dimer interface region of Corynebacterium callunae starch phosphorylase perturbs the phosphate-dependent conformational relay that enhances oligomeric stability of the enzyme.

Nidetzky, Bernd; Griessler, Richard; Pierfederici, Francesco-Maria; Psik, Barbara; Sciré, Andrea; Tanfani, Fabio

Nidetzky, Bernd; Griessler, Richard; Pierfederici, Francesco-Maria; Psik, Barbara; Sciré, Andrea; Tanfani, Fabio.

Affiliation

Nidetzky B; Institute of Biotechnology, Graz University of Technology, Petersgasse 12/I, A-8010 Graz, Austria. bernd.nidetzky@tugraz.at

J Biochem ; 134(4): 599-606, 2003 Oct.

Article in En | MEDLINE | ID: mdl-14607988

Subject(s)

Corynebacterium/enzymology; Starch Phosphorylase/chemistry; Alanine/chemistry; Amino Acid Sequence; Dimerization; Hydrogen Peroxide/pharmacology; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phosphates/chemistry; Phosphates/metabolism; Plasmids/metabolism; Protein Conformation; Protein Denaturation; Protein Structure, Quaternary; Spectrophotometry, Infrared; Spectroscopy, Fourier Transform Infrared; Temperature

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Collection: 01-internacional Database: MEDLINE Main subject: Corynebacterium / Starch Phosphorylase Language: En Journal: J Biochem Year: 2003 Document type: Article Affiliation country: Austria Country of publication: United kingdom

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